Rekombinanter mTagBFP Antikörper (DBCO)

Produktnummer ABIN7272957

Der Alpaca Monoklonal Anti--Antikörper wurde für FACS, ICC und IF validiert. Er ist geeignet, in Proben von Entacmaea quadricolor zu detektieren.

Kurzübersicht für Rekombinanter mTagBFP Antikörper (DBCO) (ABIN7272957)

Target: mTagBFP

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Entacmaea quadricolor

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: DBCO

Applikation: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 1H7

Produktdetails

Expressionssystem: E.coli

Spezifität: Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2, mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657

Aufreinigung: Produced in: E.coli

Immunogen: Our sdAb binds strongly to TagRFP, Tag BFP and mTagBFP2.

Anwendungsinformationen

Applikationshinweise: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Antigendetails

Target: mTagBFP

Hintergrund: TagRFP was engineered in 2007 from TurboRFP (derived from Entacmaea quadricolor). Tag RFP displayed an extended fluorescence lifetime and showed a robust photostability. In 2008 after further mutagenesis and the exchange of ~12 amino acid residues, this red-emitting protein turned into a blue-emitting protein TagBFP. Only several years later, in 2011 it a monomeric version of TagBFP was discribed: mTagBFP2.